Z700401
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Z700401
Sigma
Manipulating the Mouse Embryo: A Laboratory Manual, 3rd ed.
| ISBN-10: | 0-87969-591-9 |
| ISBN-13: | 978-0-87969-591-0 |
Description
| General description | Regarded as the "Bible" of mammalian embryo manipulation techniques, the third edition has been completely reorganized and rewritten. The result is a compilation of new, cutting-edge protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitro fertilization), whole embryo culture systems, electroporation, embryo and gamete cryopreservation and rederivation, and gene expression, as well as more extensive background information on the use of these techniques. |
Properties
| publication info | A. Nagy, et al., Cold Spring Harbor Laboratory Press, 2003, 764 pp., soft cover |
Table Of Contents
| Table of Contents | Chapter 1. Developmental Genetics and Embryology of the Mouse: Past, Present, and Future Chapter 2. Summary of Mouse Development Chapter 3. Production of Transgenic and Chimeric Mice: General Issues Chapter 4. Recovery and In Vitro Culture of Pre-implantation–Stage Embryos Chapter 5. Isolation, Culture, and Manipulation of Post-implantation Embryos Chapter 6. Surgical Procedures Chapter 7. Production of Transgenic Mice Chapter 8. Isolation, Culture, and Manipulation of Blastocyst–derived Stem Cells Chapter 9. Vector Designs for Embryonic Stem Cell-based Transgenesis and Genome Alterations Chapter 10. Introduction of Foreign DNA into Embryonic Stem Cells Chapter 11. Production of Chimeras Chapter 12. Detection and Analysis of Mouse Genome Alterations and Specific Sequences Chapter 13. Parthenogenesis, Pronuclear Transfer, and Mouse Cloning Chapter 14. Assisted Reproduction: Ovary Transplantation, In Vitro Fertilization, Artificial Insemination, and Intracytoplasmic Sperm Injection Chapter 15. Cryopreservation, Rederivation, and Transport of Mice Chapter 16. Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems Chapter 17. Setting up and Equipping a Micromanipulation Lab Appendices |
